Ampha Z40 – Pollen Analyzer

Accurate, fast, and easy pollen count and viability analysis

Manufacturer: Amphasys AG, Switzerland


The Ampha Z40 Impedance Flow Cytometer can be used for pollen quality analysis for: 

  • Increased yield in seed production
  • Increased reliability in seed production
  • Lower seed production costs

 Knowing how your genetics with perform can give you the possibility of identifying best pollinator lines in an early stage, breed for higher heat resistance, and optimize female to male ratios. 

Benefits of the Ampha Z40

Universal: Measure Pollen of All Crops 

The Z40 Ampha Pollen Analyzer measures pollen of all relevant plant species, ranging from row crops and vegetables to trees and ornamentals. We provide support and detailed application recommendations for more than 250 species. 

The Z40 can also be used as an easy tool to determine pollen ploidy, or developmental stages of microspores. Being able to identify the right developmental stage for DH production can significantly increase the output of embryos and makes it more efficient. 

Efficient: Acquire and Share Standardized Pollen Information from Breeding to Seed Production

The Ampha Z40 provides information which can be used in all stages, from early research in plant breeding up to commercial seed production: 

  • Improve doubled haploid (DH) plant production
  • Line selection breeding
  • Optimizing pollen storage, pollination protocols, and female to male ratio
  • Routine pollen quality control in commercial seed production

Full Flexibility: From Research to Routine

The Ampha Z40 is the high-end pollen analyzer which covers all requirements for research, as well as for routine measurements. Protocols and settings can be adapted in a flexible way to match requirements of specific research topics, which makes it an ideal tool for in-depth analyses: 

  • Creation of own protocols
  • Manual gating strategies
  • Free variation of settings
  • Adaptation of buffer solutions

Pollen Viability Analysis with the Ampha Z40 Pollen Analyzer 

The Ampha Z40 Pollen Analyzer is the successor of the Ampha Z32, dedicated for pollen analysis: 

  • Pollen viability
  • Pollen concentration
  • Microscope developmental stages
  • Pollen ploidy

Key features of this new device are: 


The Z40 uses AmphaSoft software to intuitively combine intrument operation and data acquisition, and analysis for routine viability monitoring. 

Download the software

Advanced features include:

  • Flexible gate settings
  • Overlays to compare measurements 
  • Data export in csv and html format

Benefits of Pollen Analysis Along Your Value Chain


With identification of the developmental stage of microspores you can select only these microspores which are appropriate for use for DH production. It is even possible to predict the yield of the embryos which turns your DH production much more efficient. 


Pollen quality is an important parameter in plant breeding as it provides information about the impact of heat stress and other environmental effects on lines and allows an early elimination of bad pollinator lines. 

Production Research

In production research the Ampha Z40 is the tool to improve efficiency: with the optimization of the female to male ratio highly valuable space can be saved, the knowledge about the right pollen viability threshold helps to produce only the necessary amount of pollen and to develop pollen dilution formulations. Furthermore, the development of pollen storage protocols secures a permanent stock of high-quality pollen. 

Seed Production

In seed production this technology allows routine checks of pollen quality, and prevents the loss of highly valuable pollen. This can be done to check if the pollen viability has still sufficient viability before harvesting or before using it for pollination. Especially if pollen is shipped between locations, the Amphasys technology secures a fast and accurate quality check before and after shipping – independent from the location. 

Pollen Expert Blog

Double Haploid Plant Production – March 23, 2021

Overcoming the Pollination Gamble with Science – November 19, 2020

Measuring Wheat Pollen Viability – A Delicate Procedure?! – October 20, 2020

How Pollen Quality Affects Seed Set – September 22, 2020

Unravelling Hidden Secrets Behind Dead Tomato Pollen – June 4, 2020

Impact of Pollen Quality on Tomato Seed Production – February 28, 2020

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Amphasys Z40 Brochure
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