| Most of the
Protocols presets we offer with the Eddy Jet Spiral Plater are industry
standards that were developed by various manufacturers in the 15-20 years since
this method became a common method. By offering all
the different protocols out there, user of “older units” would not have to do a
lengthy validation process when they upgraded to the Eddy Jet, rather, use their
existing protocol which was programmed into the memory of the unit.
Some of the protocols use exponential or logarithmic distribution of the sample
on the plate, so that a 3 fold dilution (1-1000) will be forming on a 100mm
plate. The linear and lawn protocols, however do not get that dilution rather offer an
ever distribution on the plate. Again, the different protocol names, (C, D…) and volumes (49.2, 50 100ul) can be
used to your choice with no real preference, thus the most important to
remember, is to read the plates using the exact protocol (if using automation
i.e. with our Flash & Grow colony counter ) or with the right volumes of liquids
calculated in the formulas as presented the instruction manual (when using
manual readings). |
